解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::The identification of specific protein markers for colorectal cancer would provide the basis for early diagnosis and detection, as well as clues for understanding the molecular mechanisms governing cancer progression. In this report, we describe the proteomic analysis of the samples of colorectal cancer corresponding ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200401196
更新日期:2005-07-01 00:00:00
abstract::Carcinoma of the prostate (CaP) is the second leading cause of cancer-related mortality among American men. While high cure rates are associated with localized CaP, no cure exists for advanced recurrent disease. At present there are no known serologic biomarkers specific to this stage of the disease. Several groups ha...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200401165
更新日期:2005-07-01 00:00:00
abstract::Prior to analysis by mass spectrometry, protein samples are often digested. Maximizing the peptide yield from digestion can increase the number of peptides detected and the confidence in protein identification. To determine the optimal conditions for digestion, the Michaelis-Menten kinetic parameters for Promega seque...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200401268
更新日期:2005-06-01 00:00:00
abstract::The ectopic expression of the gene SEL1L in the human breast carcinoma cell line MCF-7 resulted in a reduction of the aggressive behaviour of these cells in vitro. In addition, in vivo analysis on a series of primary breast carcinomas revealed an association between the SEL1L protein levels and the patient's overall s...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200401283
更新日期:2005-06-01 00:00:00
abstract::Comparative proteome profiling using stable isotope peptide labelling and mass spectrometry has emerged as a promising strategy. Here, we show the broad potential of our proprietary protein sequence tag (PST) technology. A special feature of PST is its ability to detect a wide variety of proteins including the pharmac...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200401306
更新日期:2005-06-01 00:00:00
abstract::Matrix-assisted laser desorption/ionization mass spectrometry and tandem mass spectrometry (MS/MS) were used to determine the multiple acetylation sites in the histone acetyltransferase (HAT): p300-HAT. Partial cleavage of the peptides containing acetylated lysine residues by trypsin provided a set of nested sequences...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200401167
更新日期:2005-06-01 00:00:00
abstract::In this study, we generated 40 somatic cell cloned (scNT) piglets. Of these, five piglets were stillborn, 22 scNT piglets died suddenly within the first week of life, and 1 piglet died after 40 days. Twelve scNT piglets are still healthy. The birth weights of compromised scNT piglets in comparison with those of normal...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200401079
更新日期:2005-05-01 00:00:00
abstract::PROTICdb is a web-based application, mainly designed to store and analyze plant proteome data obtained by two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) and mass spectrometry (MS). The purposes of PROTICdb are (i) to store, track, and query information related to proteomic experiments, i.e., from tissue...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200401111
更新日期:2005-05-01 00:00:00
abstract::Non-DNA microarrays, such as protein, peptide and small molecule microarrays, can potentially revolutionize the high-throughput screening tools currently used in basic and pharmaceutical research. However, fundamental obstacles remain that limit their rapid and widespread implementation as an alternative bioanalytical...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200401078
更新日期:2005-05-01 00:00:00
abstract::The basic problem of complexity poses a significant challenge for proteomic studies. To date two-dimensional gel electrophoresis (2-DE) followed by enzymatic in-gel digestion of the peptides, and subsequent identification by mass spectrometry (MS) is the most commonly used method to analyze complex protein mixtures. H...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200401122
更新日期:2005-05-01 00:00:00
abstract::Proteome analysis of human hepatocellular carcinoma tissues was conducted using two-dimensional difference gel electrophoresis coupled with mass spectrometry. Paired samples from the normal and tumor region of resected human liver were labeled with Cy3 and Cy5, respectively while the pooled standard sample was labeled...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200401256
更新日期:2005-05-01 00:00:00
abstract::In the postgenomic era, many researchers and organizations have been engaged in structural and functional analyses of proteins. As a part of these efforts, searching for small organic compounds that bind specifically to target proteins is quite important. In this study, we have developed a rational strategy for ligand...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200401032
更新日期:2005-04-01 00:00:00
abstract::High-density lipoprotein (HDL) is the most abundant lipoprotein particle in the plasma and a negative risk factor of atherosclerosis. By using a proteomic approach it is possible to obtain detailed information about its protein content and protein modifications that may give new information about the physiological rol...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200401010
更新日期:2005-04-01 00:00:00
abstract::Automated multidimensional capillary liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been increasingly applied in various large scale proteome profiling efforts. However, comprehensive global proteome analysis remains technically challenging due to issues associated with sample complexity and dynamic ran...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200401055
更新日期:2005-04-01 00:00:00
abstract::Helicobacter pylori is one of the most common bacterial pathogens and causes a variety of diseases, such as peptic ulcer or gastric cancer. Despite intensive study of this human pathogen in the last decades, knowledge about its membrane proteins and, in particular, those which are putative components of the type IV se...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200401019
更新日期:2005-04-01 00:00:00
abstract::Human saliva contains a large number of proteins and peptides (salivary proteome) that help maintain homeostasis in the oral cavity. Global analysis of human salivary proteome is important for understanding oral health and disease pathogenesis. In this study, large-scale identification of salivary proteins was demonst...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200401037
更新日期:2005-04-01 00:00:00
abstract::Tumor development and progression consist of a series of complex processes involving multiple changes in gene expression (Paolo et al. Physiol. Rev., 1993, 73, 161-195; Lance et al. Cell., 1991, 64, 327-336). Tumor cells acquire an invasive and metastatic phenotype that is the main cause of death for cancer patients. ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200401132
更新日期:2005-03-01 00:00:00
abstract::The CpG motif of bacterial DNA (CpG-DNA) is a potent immunostimulating agent whose mechanism of action is not yet clear. Here, we used both DNA microarray and proteomic approaches to investigate the effects of oligodeoxynucleotides containing the CpG motif (CpG-ODN) on gene transcription and protein expression profile...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200401144
更新日期:2005-03-01 00:00:00
abstract::Blood plasma and serum are very useful samples for the detection, identification and quantitation of proteins associated with both health and disease. However, analysis of plasma and serum is a challenge because traces of interesting polypeptides and proteins can be dominated by the very high concentration of albumin ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200401065
更新日期:2005-03-01 00:00:00
abstract::In higher plants many different genes encode Lhcb proteins that belong to a highly conserved protein family. Evolutionary conservation of this genetic redundancy suggests that individual gene products play different roles in light harvesting and photoprotection depending on environmental conditions. We have tested the...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200402008
更新日期:2005-02-01 00:00:00
abstract::Myostatin plays a major role in muscle growth and development and animals with disruption of this gene display marked increases in muscle mass. Little is known about muscle physiological adaptations in relation to this muscle hypertrophy. To provide a more comprehensive view, we analyzed bovine muscles from control, h...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200400925
更新日期:2005-02-01 00:00:00
abstract::We have combined several key sample preparation steps for the use of a liquid matrix system to provide high analytical sensitivity in automated ultraviolet -- matrix-assisted laser desorption/ionisation -- mass spectrometry (UV-MALDI-MS). This new sample preparation protocol employs a matrix-mixture which is based on ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200400956
更新日期:2005-02-01 00:00:00
abstract::The present review attempts to cover a number of methods that have appeared in the last few years for performing quantitative proteome analysis. However, due to the large number of methods described for both electrophoretic and chromatographic approaches, we have limited this review to conventional two-dimensional (2-...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.200401015
更新日期:2005-02-01 00:00:00
abstract::The phosphorylation of heat shock protein 27 (HSP27) occurs differently in human renal cell carcinoma (RCC) compared to homologous normal kidney tissue. Two-dimensional electrophoresis was used to separate and visualize HSP27, via immunostaining with anti-HSP27 antibody, in tumor and normal renal samples, obtained aft...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200401134
更新日期:2005-02-01 00:00:00
abstract::We have combined high-resolution two-dimensional (2-D) gel electrophoresis and mass spectrometry with the aim of identifying proteins represented in the 2-D gel database of the wing imaginal discs of Drosophila melanogaster. First, we obtained a high-resolution 2-D gel pattern of [35S]methionine + [35S]cysteine-labele...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200400923
更新日期:2005-02-01 00:00:00
abstract::The severe pulmonary form of leptospirosis (SPFL) is an especially serious and rapid disease process characterized by alveolar hemorrhage and acute respiratory failure. The outer membrane of Leptospira facilitates direct interactions with the environs and likely contains important constituents involved during infectio...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200400880
更新日期:2005-01-01 00:00:00
abstract::Acceleration of liver regeneration could be of great clinical benefit in various liver-associated diseases. However, at present little is known about therapeutic interventions to enhance this regenerative process. Our limited understanding and the complexity of the mechanisms involved have prevented the identification...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200400913
更新日期:2005-01-01 00:00:00
abstract::Proteome analysis by two-dimensional gel electrophoresis (2-DE) together with mass spectrometry was applied to screen acute phase response (APR)-related proteins with low molecular weight in loach skin following injury. Furthermore, Western blotting and function tests were applied to confirm the results obtained from ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300864
更新日期:2004-12-01 00:00:00
abstract::Treatment of patients with malignant melanoma with interferon-alpha achieves a response in a small but significant subset of patients. Currently, although much is known about interferon biology, little is known about either the particular mechanisms of interferon-alpha activity that are crucial for response or why onl...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200400870
更新日期:2004-12-01 00:00:00
abstract::Separation and identification of hydrophobic membrane proteins is a major challenge in proteomics. Identification of such sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)-separated proteins by peptide mass fingerprinting (PMF) via matrix-assisted laser desorption/ionization-time of flight (MALDI-TO...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200400851
更新日期:2004-12-01 00:00:00
abstract::The activation of caspases is a critical event for the execution phase of programmed cell death. Caspases are highly specific in their ability to activate or inhibit many crucial proteins in the cell via site-specific cleavage. To date, more than 60 proteins have been shown to be substrates of one or more caspases in ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200400979
更新日期:2004-11-01 00:00:00
abstract::Atopic dermatitis (AD) is a chronic relapsing inflammatory skin disease typically characterized by a distribution of eczematous skin lesions with lichenification, pruritic excoriations, and dry skin with wide varieties of pathophysiologic aspects. Recently, AD was divided into extrinsic and intrinsic forms according t...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200400998
更新日期:2004-11-01 00:00:00
abstract::Heat shock proteins (HSPs) are crucial for the maintenance of cell integrity during normal cellular growth as well as during pathophysiological conditions. While functioning mainly as molecular chaperones, HSPs also appear to be involved in diverse biological activities, such as apoptosis, carcinogenesis, and cytoprot...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200400951
更新日期:2004-11-01 00:00:00
abstract::Intracellular serine protease-1 (Isp-1) is a major intracellular serine protease of Bacillus subtilis, whose functions still remain largely unknown. Furthermore, physiological substrates are yet to be determined. To identify Isp-1 substrates, we digested extract obtained from an Isp-1 deficient Bacillus mutant with pu...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200400997
更新日期:2004-11-01 00:00:00
abstract::ESAT-6 (the 6 kDa early secreted antigenic target) protein species in short-term culture filtrate of Mycobacterium tuberculosis were separated in a 4-5 narrow range pI gradient two-dimensional gel electrophoresis (2-DE). Eight ESAT-6 protein species were analyzed in detail by peptide mass fingerprinting matrix-assiste...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200400906
更新日期:2004-10-01 00:00:00
abstract::The surface subproteome of Listeria monocytogenes that includes many proteins already known to be involved in virulence and interaction with host cells has been characterized. A new method for the isolation of a defined surface proteome of low complexity has been established based on serial extraction of proteins by d...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200400928
更新日期:2004-10-01 00:00:00
abstract::The ability to obtain the accurate mass of a protein in a complex sample mixture aids in determining its correct in vivo form. This is important when identifying post-translationally modified proteins, protein variants or isoforms. The central technique used to separate proteins, 2-dimensional gel electrophoresis offe...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200300843
更新日期:2004-10-01 00:00:00
abstract::Since drug resistance is a complex and multifactorial event involving activation/repression of multiple biochemical pathways, we used a proteomic approach to study cisplatin resistance and drug response in human tumor cell lines. The cervix squamous cell carcinoma cell line A431 and its cisplatin-resistant subline, A4...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200400835
更新日期:2004-10-01 00:00:00
abstract::In an effort to identify tumor-associated proteins from plasma of tumor-bearing mice that may be used as diagnostic biomarkers, we developed a strategy that combines a tumor xenotransplantation model in nude mice with comparative proteomic technology. Five human cancer cell lines (SC-M1, HONE-1, CC-M1, OECM1, GBM 8401...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200400785
更新日期:2004-09-01 00:00:00
abstract::The use of anthrax spores as a bioweapon has spurred efforts aimed at identifying key proteins expressed in Bacillus anthracis. Because spore germination and outgrowth occur prior to and are required for disease manifestations, blocking germination and early outgrowth with novel vaccines or inhibitors targeting critic...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200400831
更新日期:2004-09-01 00:00:00